Identification of Ribonucleoprotein (RNP)-Specific Protein Interactions Using a Yeast RNP Interaction Trap Assay (RITA)
نویسندگان
چکیده
منابع مشابه
Identification of ribonucleoprotein (RNP)-specific protein interactions using a yeast RNP interaction trap assay (RITA).
We describe an adaptation of the yeast three-hybrid system that allows the reconstitution in vivo of tripartite (protein-RNA-protein) ribonucleoproteins (RNPs). To build and try this system that we called RNP interaction trap assay (RITA), we used as a model the autoantigenic Ro RNPs. hY RNAs bear distinct binding sites for Ro60 and La proteins, and Ro RNPs are thus physiologically tripartite (...
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Spinal muscular atrophy is a common, often lethal, neurodegenerative disease that results from low levels of, or loss-of-function mutations in, the SMN (survival of motor neurons) protein. SMN oligomerizes and forms a stable complex with five additional proteins: Gemins 2–6. SMN also interacts with several additional proteins referred to as ‘substrates’. Most of these substrates contain a domai...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99274rr02